Homozygous splice mutation in CWF19L1 in a Turkish family with recessive ataxia syndrome
Identifieur interne : 000A46 ( Main/Exploration ); précédent : 000A45; suivant : 000A47Homozygous splice mutation in CWF19L1 in a Turkish family with recessive ataxia syndrome
Auteurs : Randi Burns ; Karen Majczenko ; Jishu Xu ; Weiping Peng ; Zuhal Yapici ; James J. Dowling ; Jun Z. Li ; Margit BurmeisterSource :
- Neurology [ 0028-3878 ] ; 2014.
Abstract
To elucidate the genetic cause of a rare recessive ataxia presented by 2 siblings from a consanguineous Turkish family with a nonprogressive, congenital ataxia with mental retardation of unknown etiology.
Whole-exome sequencing was combined with homozygosity mapping, linkage, and expression analysis to identify candidate genes, confirmed by Sanger sequencing. Reverse transcription–PCR and immunoblotting were used to determine the functional consequences of the gene variant. A zebrafish model was developed using morpholino-mediated knockdown.
We identified a homozygous mutation at the invariant +1 position (c.964+1G>A) in intron 9 of the
Our results suggest that
Url:
DOI: 10.1212/WNL.0000000000001053
PubMed: 25361784
PubMed Central: 4276403
Affiliations:
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in a Turkish family with recessive ataxia syndrome</title>
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<author><name sortKey="Majczenko, Karen" sort="Majczenko, Karen" uniqKey="Majczenko K" first="Karen" last="Majczenko">Karen Majczenko</name>
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<author><name sortKey="Yapici, Zuhal" sort="Yapici, Zuhal" uniqKey="Yapici Z" first="Zuhal" last="Yapici">Zuhal Yapici</name>
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<author><name sortKey="Li, Jun Z" sort="Li, Jun Z" uniqKey="Li J" first="Jun Z." last="Li">Jun Z. Li</name>
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<author><name sortKey="Burmeister, Margit" sort="Burmeister, Margit" uniqKey="Burmeister M" first="Margit" last="Burmeister">Margit Burmeister</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Homozygous splice mutation in <italic>CWF19L1</italic>
in a Turkish family with recessive ataxia syndrome</title>
<author><name sortKey="Burns, Randi" sort="Burns, Randi" uniqKey="Burns R" first="Randi" last="Burns">Randi Burns</name>
</author>
<author><name sortKey="Majczenko, Karen" sort="Majczenko, Karen" uniqKey="Majczenko K" first="Karen" last="Majczenko">Karen Majczenko</name>
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<author><name sortKey="Xu, Jishu" sort="Xu, Jishu" uniqKey="Xu J" first="Jishu" last="Xu">Jishu Xu</name>
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<author><name sortKey="Peng, Weiping" sort="Peng, Weiping" uniqKey="Peng W" first="Weiping" last="Peng">Weiping Peng</name>
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<author><name sortKey="Yapici, Zuhal" sort="Yapici, Zuhal" uniqKey="Yapici Z" first="Zuhal" last="Yapici">Zuhal Yapici</name>
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<author><name sortKey="Dowling, James J" sort="Dowling, James J" uniqKey="Dowling J" first="James J." last="Dowling">James J. Dowling</name>
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<author><name sortKey="Li, Jun Z" sort="Li, Jun Z" uniqKey="Li J" first="Jun Z." last="Li">Jun Z. Li</name>
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<author><name sortKey="Burmeister, Margit" sort="Burmeister, Margit" uniqKey="Burmeister M" first="Margit" last="Burmeister">Margit Burmeister</name>
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<series><title level="j">Neurology</title>
<idno type="ISSN">0028-3878</idno>
<idno type="eISSN">1526-632X</idno>
<imprint><date when="2014">2014</date>
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<front><div type="abstract" xml:lang="en"><sec><title>Objective:</title>
<p>To elucidate the genetic cause of a rare recessive ataxia presented by 2 siblings from a consanguineous Turkish family with a nonprogressive, congenital ataxia with mental retardation of unknown etiology.</p>
</sec>
<sec><title>Methods:</title>
<p>Whole-exome sequencing was combined with homozygosity mapping, linkage, and expression analysis to identify candidate genes, confirmed by Sanger sequencing. Reverse transcription–PCR and immunoblotting were used to determine the functional consequences of the gene variant. A zebrafish model was developed using morpholino-mediated knockdown.</p>
</sec>
<sec><title>Results:</title>
<p>We identified a homozygous mutation at the invariant +1 position (c.964+1G>A) in intron 9 of the <italic>CWF19L1</italic>
(complexed with cdc5 protein 19-like 1) gene. This mutation is absent in >6,500 European and African American individuals and 200 Turkish control DNAs. The mutation causes exon skipping, reduction in messenger RNA levels, and protein loss in cell lines of affected individuals. Morpholino-mediated knockdown in a zebrafish model demonstrates that loss of the evolutionarily highly conserved <italic>CWF19L1</italic>
, whose normal biological function is unknown, alters cerebellar morphology and causes movement abnormalities.</p>
</sec>
<sec><title>Conclusions:</title>
<p>Our results suggest that <italic>CWF19L1</italic>
mutations may be a novel cause of recessive ataxia with developmental delay. Our research may help with diagnosis, especially in Turkey, identify causes of other ataxias, and may lead to novel therapies.</p>
</sec>
</div>
</front>
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<tree><noCountry><name sortKey="Burmeister, Margit" sort="Burmeister, Margit" uniqKey="Burmeister M" first="Margit" last="Burmeister">Margit Burmeister</name>
<name sortKey="Burns, Randi" sort="Burns, Randi" uniqKey="Burns R" first="Randi" last="Burns">Randi Burns</name>
<name sortKey="Dowling, James J" sort="Dowling, James J" uniqKey="Dowling J" first="James J." last="Dowling">James J. Dowling</name>
<name sortKey="Li, Jun Z" sort="Li, Jun Z" uniqKey="Li J" first="Jun Z." last="Li">Jun Z. Li</name>
<name sortKey="Majczenko, Karen" sort="Majczenko, Karen" uniqKey="Majczenko K" first="Karen" last="Majczenko">Karen Majczenko</name>
<name sortKey="Peng, Weiping" sort="Peng, Weiping" uniqKey="Peng W" first="Weiping" last="Peng">Weiping Peng</name>
<name sortKey="Xu, Jishu" sort="Xu, Jishu" uniqKey="Xu J" first="Jishu" last="Xu">Jishu Xu</name>
<name sortKey="Yapici, Zuhal" sort="Yapici, Zuhal" uniqKey="Yapici Z" first="Zuhal" last="Yapici">Zuhal Yapici</name>
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